TY - JOUR
T1 - Enhancement of damage-specific dna binding of xpa by interaction with the ERCC1 DNA repair protein
AU - Nagai, Akira
AU - Saijo, Masafumi
AU - Kuraoka, Isao
AU - Matsuda, Toshiro
AU - Kodo, Naohiko
AU - Nakatsu, Yoshimichi
AU - Mimaki, Takashi
AU - Mino, Makoio
AU - Biggerstaff, Maureen
AU - Wood, Richard D.
AU - Sijbers, Anneke
AU - Hoeijmakers, Jan H.J.
AU - Tanaka, Kiyoji
PY - 1995/6/26
Y1 - 1995/6/26
N2 - The human XPA and ERCC1 proteins, which are involved in early steps of nucleotide excision repair of DNA, specifically interacted in an in vitro binding assay and a yeast two-hybrid assay. A stretch of consecutive glutamic acid residues in XPA was needed for binding to ERCC1. Binding of XPA to damaged DNA was markedly increased by the interaction of the XPA and ERCC1 proteins. ERCC1 did not enhance binding to DNA when a truncated XPA protein, MF122, was used in place of the XPA protein. MF122 retains damaged DNA binding activity but lacks the region for protein-protein interaction including the E-cluster region. These results suggest that the XPA/ERCC1 interaction may participate in damage-recognition as well as in incision at the 5′ site of damage during nucleotide excision repair.
AB - The human XPA and ERCC1 proteins, which are involved in early steps of nucleotide excision repair of DNA, specifically interacted in an in vitro binding assay and a yeast two-hybrid assay. A stretch of consecutive glutamic acid residues in XPA was needed for binding to ERCC1. Binding of XPA to damaged DNA was markedly increased by the interaction of the XPA and ERCC1 proteins. ERCC1 did not enhance binding to DNA when a truncated XPA protein, MF122, was used in place of the XPA protein. MF122 retains damaged DNA binding activity but lacks the region for protein-protein interaction including the E-cluster region. These results suggest that the XPA/ERCC1 interaction may participate in damage-recognition as well as in incision at the 5′ site of damage during nucleotide excision repair.
UR - http://www.scopus.com/inward/record.url?scp=0029061081&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.1905
DO - 10.1006/bbrc.1995.1905
M3 - Article
C2 - 7598728
AN - SCOPUS:0029061081
SN - 0006-291X
VL - 211
SP - 960
EP - 966
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -