TY - JOUR
T1 - ENU mutagenesis to generate genetically modified rat models.
AU - van Boxtel, Ruben
AU - Gould, Michael N.
AU - Cuppen, Edwin
AU - Smits, Bart M.G.
PY - 2010
Y1 - 2010
N2 - The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
AB - The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
UR - http://www.scopus.com/inward/record.url?scp=77449094451&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-389-3_11
DO - 10.1007/978-1-60327-389-3_11
M3 - Article
C2 - 20013232
AN - SCOPUS:77449094451
SN - 1940-6029
VL - 597
SP - 151
EP - 167
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -