EpCAM-based flow cytometry in cerebrospinal fluid greatly improves diagnostic accuracy of leptomeningeal metastases from epithelial tumors

Bojana Milojkovic Kerklaan, Dick Pluim, Mijke Bol, Ingrid Hofland, Johan Westerga, Harm Van Tinteren, Jos H. Beijnen, Willem Boogerd, Jan H.M. Schellens, Dieta Brandsma

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

48 Citaten (Scopus)

Samenvatting

Background Moderate diagnostic accuracy of MRI and initial cerebrospinal fluid (CSF) cytology analysis results in at least 10%-15% false negative diagnoses of leptomeningeal metastases (LM) of solid tumors, thus postponing start of therapy. The aim of this prospective clinical study was to determine the diagnostic value of epithelial cell adhesion molecule (EpCAM)-based flow cytometry versus cytology in CSF for the diagnosis of LM in patients with epithelial tumors. Methods Patients with a clinical suspicion of LM but a negative or inconclusive MRI in whom a diagnostic lumbar puncture has to be performed were included. At least 5 mL of CSF for cytology, 5 mL for flow cytometry, 2 mL for cell count and biochemistry, and 8 mL whole blood samples for circulating tumor cells measurements and biochemistry were drawn. Tumor cells in CSF and whole blood were detected by multiparameter flow cytometry using EpCAM antibody. Results In total 29 eligible patients were enrolled in the study. Thirteen patients were ultimately diagnosed with LM. The flow cytometry assay showed 100% sensitivity and 100% specificity for diagnosing LM, while sensitivity of CSF cytology was only 61.5%. Cell count or biochemical parameters in CSF were abnormal in 100% of patients with LM. Conclusions Our results suggest that the EpCAM-based flow cytometry assay is superior to CSF cytology for the diagnosis of LM in patients with an epithelial tumor, a clinical suspicion of LM, and a nonconclusive MRI. Confirmation of these data is needed in a larger dataset to recommend dual CSF diagnostics for LM. ClinicalTrials.gov Identifier NCT01713699.

Originele taal-2Engels
Pagina's (van-tot)855-862
Aantal pagina's8
TijdschriftNeuro-Oncology
Volume18
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 10 jun. 2016
Extern gepubliceerdJa

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