Epigenetic reader complexes of the human malaria parasite, Plasmodium falciparum

Wieteke Anna Maria Hoeijmakers, Jun Miao, Sabine Schmidt, Christa Geeke Toenhake, Sony Shrestha, Jeron Venhuizen, Rob Henderson, Jakob Birnbaum, Sonja Ghidelli-Disse, Gerard Drewes, Liwang Cui, Hendrik Gerard Stunnenberg, Tobias Spielmann, Richárd Bártfai

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

25 Citaten (Scopus)

Samenvatting

Epigenetic regulatory mechanisms are central to the development and survival of all eukaryotic organisms. These mechanisms critically depend on the marking of chromatin domains with distinctive histone tail modifications (PTMs) and their recognition by effector protein complexes. Here we used quantitative proteomic approaches to unveil interactions between PTMs and associated reader protein complexes of Plasmodium falciparum, a unicellular parasite causing malaria. Histone peptide pull-downs with the most prominent and/or parasite-specific PTMs revealed the binding preference for 14 putative and novel reader proteins. Amongst others, they highlighted the acetylation-level-dependent recruitment of the BDP1/BDP2 complex and identified an PhD-finger protein (PHD 1, PF3D7_1008100) that could mediate a cross-talk between H3K4me2/3 and H3K9ac marks. Tagging and interaction proteomics of 12 identified proteins unveiled the composition of 5 major epigenetic complexes, including the elusive TBP-associated-factor complex as well as two distinct GCN5/ADA2 complexes. Furthermore, it has highlighted a remarkable degree of interaction between these five (sub)complexes. Collectively, this study provides an extensive inventory of PTM-reader interactions and composition of epigenetic complexes. It will not only fuel further explorations of gene regulation amongst ancient eukaryotes, but also provides a stepping stone for exploration of PTM-reader interactions for antimalarial drug development.

Originele taal-2Engels
Pagina's (van-tot)11574-11588
Aantal pagina's15
TijdschriftNucleic Acids Research
Volume47
Nummer van het tijdschrift22
DOI's
StatusGepubliceerd - 16 dec. 2019

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