Epigenetics and Control of RNAs

Henrike Maatz, Sebastiaan van Heesch, Franziska Kreuchwig, Allison Faber, Eleonora Adami, Norbert Hubner, Matthias Heinig

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

Histone modifications are epigenetic marks that fundamentally impact the regulation of gene expression. Integrating histone modification information in the analysis of gene expression traits (eQTL mapping) has been shown to significantly enhance the prediction of eQTLs. In this chapter, we describe (1) how to perform quantitative trait locus (QTL) analysis using histone modification levels as traits and (2) how to integrate these data with information on RNA expression for the elucidation of the epigenetic control of transcript levels. We will provide a comprehensive introduction into the topic, describe in detail how ChIP-seq data are analyzed and elaborate on how to integrate ChIP-seq and RNA-seq data from a segregating disease animal model for the identification of the epigenetic control of RNA expression.

Originele taal-2Engels
Pagina's (van-tot)217-237
Aantal pagina's21
TijdschriftMethods in molecular biology (Clifton, N.J.)
Volume1488
DOI's
StatusGepubliceerd - 2017
Extern gepubliceerdJa

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