Establishment of Pancreatic Organoids from Normal Tissue and Tumors

Else Driehuis, Ana Gracanin, Robert Gerhardus Jacob Vries, Hans Clevers, Sylvia Fernández Boj

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

16 Citaten (Scopus)

Samenvatting

Establishment of patient-derived adult stem cell-based pancreatic (tumor) organoids was first described in 2015. Since then, multiple laboratories have demonstrated the robustness of this method. We recently described the generation of a pancreatic cancer biobank containing 30 well-characterized tumor organoid models. Here, we describe the applied methods in detail. Use of tumor-selective media prevents contamination with normal cells. Generated organoids can be cryopreserved and can serve as a living biobank of pancreatic cancer for biomarker identification and drug screening. For complete information on the generation and use of this protocol, please refer to Driehuis et al. (2019).

Originele taal-2Engels
Artikelnummer100192
TijdschriftSTAR Protocols
Volume1
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 18 dec. 2020

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