Expression of 5'-nucleotidase (CD73) related to other differentiation antigens in leukemias of B-cell lineage

R. Pieters, L. F. Thompson, G. J. Broekema, D. R. Huismans, G. J. Peters, S. T. Pals, K. Hahlen, A. J.P. Veerman

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

16 Citaten (Scopus)


Ecto-5' nucleotidase (5'NT; CD73) expression was studied with a monoclonal antibody (7G2) and a radiochemical assay and compared with the expression of other antigens in B-cell-lineage leukemias on cells from 100 leukemic patients and two cell lines. A B-cell origin was confirmed by the expression of CD19 and HLA-DR. Four stages of B-cell leukemias were defined: stage I (pro-B) as CD10-, cytoplasmic μ- (cμ-), surface Ig- (slg-); stage II (cALL) as CD10+/cμ-/slg-; stage III (pre-B) as CD10(+ or -)/cμ+/slg-; and stage IV (B) as CD10-/cμ-/slg+. A linear correlation was found between immunohistochemical and radiochemical determination of 5'NT (r = .86). 5'NT expression was low in T-cell leukemias and stage I, high in stages II and III, and low again in stage IV of B-cell leukemias. 5'NT expression was not related to cμ, CD20, CD21, CD22, CD34, and terminal deoxynucleotidyl transferase (TdT) expression, but was significantly related to CD10 and inversely related to κ/λ expression. However, the 5'NT activity in CD10+ leukemias (stages II and III) shows a very wide range. Within the group of CD10+ leukemias no differences were detected between 5'NT+ and 5'NT- cells in their expression of other B-cell antigens. We conclude that the place of 5'NT in leukemias corresponding to early stages of B-cell development has been characterized. 5'NT is expressed in CD10+ stages and decreases before the expression of slgs. Future studies should make clear whether a high expression of this enzyme in CD10+ stages is a normal maturation phenomenon or a malignant phenomenon.

Originele taal-2Engels
Pagina's (van-tot)488-492
Aantal pagina's5
Nummer van het tijdschrift2
StatusGepubliceerd - 1991
Extern gepubliceerdJa


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