TY - JOUR
T1 - Expression of AC133 and CD117 on candidate normal stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia
AU - Baersch, G
AU - Baumann, M
AU - Ritter, J
AU - Jürgens, H
AU - Vormoor, J
PY - 1999/12
Y1 - 1999/12
N2 - To identify residual candidate normal progenitor/stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia (ALL), expression of AC133 and CD117 was analysed on the leukaemic cell clone and on immature B-lineage-negative CD34+CD19- bone marrow cells. 10/25 patients (40%) had no detectable expression of AC133 within the leukaemic cell clone. 24/26 patients (92%) lacked expression of CD117 on the leukaemic blast cell population. In contrast, a distinct AC133-positive cell population was found in 8/8 children with AC133-negative ALL and a CD117-positive cell population could be identified in 12/12 children with CD117-negative ALL, within the CD34+CD19- progenitor/stem cell compartment. These observations provide further evidence that in B-cell precursor ALL, unlike in acute myelogenous leukaemia, it may be possible to distinguish residual normal progenitor/stem cells from the leukaemic cell clone.
AB - To identify residual candidate normal progenitor/stem cell populations in childhood B-cell precursor acute lymphoblastic leukaemia (ALL), expression of AC133 and CD117 was analysed on the leukaemic cell clone and on immature B-lineage-negative CD34+CD19- bone marrow cells. 10/25 patients (40%) had no detectable expression of AC133 within the leukaemic cell clone. 24/26 patients (92%) lacked expression of CD117 on the leukaemic blast cell population. In contrast, a distinct AC133-positive cell population was found in 8/8 children with AC133-negative ALL and a CD117-positive cell population could be identified in 12/12 children with CD117-negative ALL, within the CD34+CD19- progenitor/stem cell compartment. These observations provide further evidence that in B-cell precursor ALL, unlike in acute myelogenous leukaemia, it may be possible to distinguish residual normal progenitor/stem cells from the leukaemic cell clone.
KW - AC133 Antigen
KW - Antigens, CD
KW - Antigens, CD19/metabolism
KW - Antigens, CD34/metabolism
KW - Child
KW - Flow Cytometry
KW - Glycoproteins/metabolism
KW - Humans
KW - Leukocyte Common Antigens/metabolism
KW - Peptides/metabolism
KW - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/metabolism
KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma/metabolism
KW - Proto-Oncogene Proteins c-kit/metabolism
KW - Reverse Transcriptase Polymerase Chain Reaction/methods
KW - Stem Cells/metabolism
U2 - 10.1046/j.1365-2141.1999.01746.x
DO - 10.1046/j.1365-2141.1999.01746.x
M3 - Article
C2 - 10583262
SN - 0007-1048
VL - 107
SP - 572
EP - 580
JO - British journal of haematology
JF - British journal of haematology
IS - 3
ER -