Functional Organization of the S. cerevisiae Phosphorylation Network

Dorothea Fiedler, Hannes Braberg, Monika Mehta, Gal Chechik, Gerard Cagney, Paromita Mukherjee, Andrea C. Silva, Michael Shales, Sean R. Collins, Sake van Wageningen, Patrick Kemmeren, Frank C.P. Holstege, Jonathan S. Weissman, Michael Christopher Keogh, Daphne Koller, Kevan M. Shokat, Nevan J. Krogan

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

215 Citaten (Scopus)


Reversible protein phosphorylation is a signaling mechanism involved in all cellular processes. To create a systems view of the signaling apparatus in budding yeast, we generated an epistatic miniarray profile (E-MAP) comprised of 100,000 pairwise, quantitative genetic interactions, including virtually all protein and small-molecule kinases and phosphatases as well as key cellular regulators. Quantitative genetic interaction mapping reveals factors working in compensatory pathways (negative genetic interactions) or those operating in linear pathways (positive genetic interactions). We found an enrichment of positive genetic interactions between kinases, phosphatases, and their substrates. In addition, we assembled a higher-order map from sets of three genes that display strong interactions with one another: triplets enriched for functional connectivity. The resulting network view provides insights into signaling pathway regulation and reveals a link between the cell-cycle kinase, Cak1, the Fus3 MAP kinase, and a pathway that regulates chromatin integrity during transcription by RNA polymerase II.

Originele taal-2Engels
Pagina's (van-tot)952-963
Aantal pagina's12
Nummer van het tijdschrift5
StatusGepubliceerd - 6 mrt. 2009
Extern gepubliceerdJa


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