@article{766b1e68d9dc408bb0d9d2ff943ea9c9,
title = "Gene silencing by RNAi in mammalian cells",
abstract = "This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.",
keywords = "Electroporation, Lentiviral transduction, RNA interference, shRNA, siRNA, Transfection",
author = "Frida Ponthan and Yusoff, \{Narazah Mohd\} and Soria, \{Natalia Martinez\} and Olaf Heidenreich and Kelly Coffey",
note = "Publisher Copyright: {\textcopyright} 2015 by John Wiley \& Sons, Inc.",
year = "2015",
doi = "10.1002/0471142727.mb2602s111",
language = "English",
volume = "2015",
pages = "26.2.1--26.2.17",
journal = "Current Protocols in Molecular Biology",
issn = "1934-3639",
publisher = "John Wiley \& Sons Inc.",
}