Generation of Genetically Modified Rodents Using Random ENU Mutagenesis

Ruben van Boxtel, Edwin Cuppen

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukpeer review

5 Citaten (Scopus)


The generation of genetically modified animals using N-ethyl-N-nitrosourea (ENU) mutagenesis is a fast and highly effective method. The technique is based on treating male animals with the supermutagen ENU, which randomly introduces mutations in the spermatogonial stem cells. By breeding these animals with untreated females, an F1 population is generated in which each individual carries unique random ENU-induced mutations, which can be retrieved using either genotype-driven or phenotype-driven approaches. No complicated cell culturing techniques are required and since no foreign DNA is introduced, the mutant animals that are generated are not transgenic. Here, we describe the detailed protocols for ENU mutagenesis and for mutant retrieval.

Originele taal-2Engels
TitelMethods in Molecular Biology
UitgeverijHumana Press Inc.
Aantal pagina's14
StatusGepubliceerd - 2011
Extern gepubliceerdJa

Publicatie series

NaamMethods in Molecular Biology
ISSN van geprinte versie1064-3745
ISSN van elektronische versie1940-6029


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