Genome-wide binding of MBD2 reveals strong preference for highly methylated loci

Roberta Menafra; Arie B. Brinkman; Filomena Matarese; Gianluigi Franci; Stefanie J.J. Bartels; Luan Nguyen; Takashi Shimbo; Paul A. Wade; Nina C. Hubner; Hendrik G. Stunnenberg

doi:10.1371/journal.pone.0099603

Genome-wide binding of MBD2 reveals strong preference for highly methylated loci

Roberta Menafra, Arie B. Brinkman, Filomena Matarese, Gianluigi Franci, Stefanie J.J. Bartels, Luan Nguyen, Takashi Shimbo, Paul A. Wade, Nina C. Hubner, Hendrik G. Stunnenberg

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

40 Citaten (Scopus)

Samenvatting

MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.

Originele taal-2	Engels
Artikelnummer	e99603
Tijdschrift	PLoS ONE
Volume	9
Nummer van het tijdschrift	6
DOI's	https://doi.org/10.1371/journal.pone.0099603
Status	Gepubliceerd - 13 jun. 2014
Extern gepubliceerd	Ja

Toegang tot document

10.1371/journal.pone.0099603

Citeer dit

@article{71b109b878b140de829040ed9bff30e7,

title = "Genome-wide binding of MBD2 reveals strong preference for highly methylated loci",

abstract = "MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.",

author = "Roberta Menafra and Brinkman, {Arie B.} and Filomena Matarese and Gianluigi Franci and Bartels, {Stefanie J.J.} and Luan Nguyen and Takashi Shimbo and Wade, {Paul A.} and Hubner, {Nina C.} and Stunnenberg, {Hendrik G.}",

year = "2014",

month = jun,

day = "13",

doi = "10.1371/journal.pone.0099603",

language = "English",

volume = "9",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "6",

}

TY - JOUR

T1 - Genome-wide binding of MBD2 reveals strong preference for highly methylated loci

AU - Menafra, Roberta

AU - Brinkman, Arie B.

AU - Matarese, Filomena

AU - Franci, Gianluigi

AU - Bartels, Stefanie J.J.

AU - Nguyen, Luan

AU - Shimbo, Takashi

AU - Wade, Paul A.

AU - Hubner, Nina C.

AU - Stunnenberg, Hendrik G.

PY - 2014/6/13

Y1 - 2014/6/13

N2 - MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.

AB - MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=84903140408&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0099603

DO - 10.1371/journal.pone.0099603

M3 - Article

C2 - 24927503

AN - SCOPUS:84903140408

SN - 1932-6203

VL - 9

JO - PLoS ONE

JF - PLoS ONE

IS - 6

M1 - e99603

ER -

Genome-wide binding of MBD2 reveals strong preference for highly methylated loci

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit