Genomic characterization of the human DNA excision repair gene ERCC-1

Marcel van Duin; Marcel H.M. Koken; Jacolien van Den Tol; Peter Ten Dijke; Hanny Odijk; Andries Westerveld; Dirk Bootsma; Jan H.J. Hoeijmakers

doi:10.1093/nar/15.22.9195

Genomic characterization of the human DNA excision repair gene ERCC-1

Marcel van Duin, Marcel H.M. Koken, Jacolien van Den Tol, Peter Ten Dijke, Hanny Odijk, Andries Westerveld, Dirk Bootsma, Jan H.J. Hoeijmakers

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of ± 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

Originele taal-2	Engels
Pagina's (van-tot)	9195-9214
Aantal pagina's	20
Tijdschrift	Nucleic Acids Research
Volume	15
Nummer van het tijdschrift	22
DOI's	https://doi.org/10.1093/nar/15.22.9195
Status	Gepubliceerd - 25 nov. 1987
Extern gepubliceerd	Ja

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@article{10ce1442ac1e4fc0bd3be87767c54be8,

title = "Genomic characterization of the human DNA excision repair gene ERCC-1",

abstract = "In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of ± 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.",

author = "{van Duin}, Marcel and Koken, {Marcel H.M.} and {van Den Tol}, Jacolien and {Ten Dijke}, Peter and Hanny Odijk and Andries Westerveld and Dirk Bootsma and Hoeijmakers, {Jan H.J.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Anneke Graus and Petra Warmerdam for technical assistance with the promoter studies. Dr. H. Okayama is kindly acknowledged for providing the cDNA library and Dr. F.J. Benham for giving the GAPDH probe. We are indebted to Rita Boucke, Tar van Os and Mirco Kuit for preparing the manuscript. This work was financially supported by EURATOM (contract no. B16-141-NL) and MEDIGON, Foundation of Medical Scientific Research in the Netherlands (contract no. 900-501-091).",

year = "1987",

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doi = "10.1093/nar/15.22.9195",

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T1 - Genomic characterization of the human DNA excision repair gene ERCC-1

AU - van Duin, Marcel

AU - Koken, Marcel H.M.

AU - van Den Tol, Jacolien

AU - Ten Dijke, Peter

AU - Odijk, Hanny

AU - Westerveld, Andries

AU - Bootsma, Dirk

AU - Hoeijmakers, Jan H.J.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Anneke Graus and Petra Warmerdam for technical assistance with the promoter studies. Dr. H. Okayama is kindly acknowledged for providing the cDNA library and Dr. F.J. Benham for giving the GAPDH probe. We are indebted to Rita Boucke, Tar van Os and Mirco Kuit for preparing the manuscript. This work was financially supported by EURATOM (contract no. B16-141-NL) and MEDIGON, Foundation of Medical Scientific Research in the Netherlands (contract no. 900-501-091).

PY - 1987/11/25

Y1 - 1987/11/25

N2 - In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of ± 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

AB - In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of ± 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

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Genomic characterization of the human DNA excision repair gene ERCC-1

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