TY - JOUR
T1 - Hydrophobic patches on protein subunit interfaces
T2 - Characteristics and prediction
AU - Lijnzaad, Philip
AU - Argos, Patrick
PY - 1997
Y1 - 1997
N2 - Hydrophobic patches, defined as clusters of neighboring apolar atoms deemed accessible on a given protein surface, have been investigated on protein subunit interfaces. The data were taken from known tertiary structures of multimeric protein complexes. Amino acid composition and preference, patch size distribution, and patch contact complementarity across associating subunits were examined and compared with hydrophobic patches found on the solvent-accessible surface of the multimeric complexes. The largest or second largest patch on the accessible surface of the entire subunit was involved in multimeric interfaces in 90% of the cases. These results should prove useful for subunit design and engineering as well as for prediction of subunit interface regions.
AB - Hydrophobic patches, defined as clusters of neighboring apolar atoms deemed accessible on a given protein surface, have been investigated on protein subunit interfaces. The data were taken from known tertiary structures of multimeric protein complexes. Amino acid composition and preference, patch size distribution, and patch contact complementarity across associating subunits were examined and compared with hydrophobic patches found on the solvent-accessible surface of the multimeric complexes. The largest or second largest patch on the accessible surface of the entire subunit was involved in multimeric interfaces in 90% of the cases. These results should prove useful for subunit design and engineering as well as for prediction of subunit interface regions.
KW - Molecular recognition
KW - Oligomeric structure
KW - Protein structure
KW - Subunit interface
UR - http://www.scopus.com/inward/record.url?scp=0030997363&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0134(199707)28:3<333::AID-PROT4>3.0.CO;2-D
DO - 10.1002/(SICI)1097-0134(199707)28:3<333::AID-PROT4>3.0.CO;2-D
M3 - Article
C2 - 9223180
AN - SCOPUS:0030997363
SN - 0887-3585
VL - 28
SP - 333
EP - 343
JO - Proteins: Structure, Function and Genetics
JF - Proteins: Structure, Function and Genetics
IS - 3
ER -