Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box

M. Van de Wetering, M. Oosterwegel, D. Dooijes, H. Clevers

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498 Citaten (Scopus)

Samenvatting

CD3-ε gene expression is controlled by a downstream T lympocyte-specific enhancer element. We report the identification of a T cell-specific transcription factor, TCF-1, binding to this element. The multimerized recognition motif of TCF-1 constituted a T cell-specific enhancer. Subsequent cloning of TCF-1 identified three splice alternatives. TCF-1 contained a single DNA-binding HMG box most closely related to similar boxes in the putative mammalian sex-determining gene SRY and in the Schizosaccharomyces pombe Mc mating type gene. TCF-1 mRNA was expressed uniquely in T lymphocyte. Upon cotransfection into non-T cells, TCF-1 could transactivate through its cognate motif. These results identify TCF-1 as a T cell-specific transcription factor, which might play a role in the establishment of the mature T cell phenotype.

Originele taal-2Engels
Pagina's (van-tot)123-132
Aantal pagina's10
TijdschriftEMBO Journal
Volume10
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 1991
Extern gepubliceerdJa

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