Identification of an immunogenic EWS-FLI1-derived HLA-DR-restricted T helper cell epitope

Friederike Meyer-Wentrup; Günther Richter; Stefan Burdach

doi:10.1080/08880010590935194

Identification of an immunogenic EWS-FLI1-derived HLA-DR-restricted T helper cell epitope

Friederike Meyer-Wentrup, Günther Richter, Stefan Burdach

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

21 Citaten (Scopus)

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Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFN_γ video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.

Originele taal-2	Engels
Pagina's (van-tot)	297-308
Aantal pagina's	12
Tijdschrift	Pediatric Hematology and Oncology
Volume	22
Nummer van het tijdschrift	4
DOI's	https://doi.org/10.1080/08880010590935194
Status	Gepubliceerd - jun. 2005
Extern gepubliceerd	Ja

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10.1080/08880010590935194

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title = "Identification of an immunogenic EWS-FLI1-derived HLA-DR-restricted T helper cell epitope",

abstract = "Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFNγ video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.",

keywords = "Dendritic cells, Ewing family tumors, Peptide epitope, Tumor vaccination",

author = "Friederike Meyer-Wentrup and G{\"u}nther Richter and Stefan Burdach",

note = "Funding Information: Received 18 August 2004; accepted 8 February 2005. This work was supported by the BMBF-Roux Research Program of the Martin-Luther-University Halle-Wittenberg, grant number FKZ 2/21, and the Deutsche Krebshilfe, grant number FKZ 70-2787-Bu3. Address correspondence to Friederike Meyer-Wentrup, MD, Children{\textquoteright}s Cancer Research Center/Biocenter and Department of Pediatrics, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany. E-mail: friederike.meyer-wentrup@medizin.uni-halle.de",

year = "2005",

month = jun,

doi = "10.1080/08880010590935194",

language = "English",

volume = "22",

pages = "297--308",

journal = "Pediatric Hematology and Oncology",

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AU - Meyer-Wentrup, Friederike

AU - Richter, Günther

AU - Burdach, Stefan

N1 - Funding Information: Received 18 August 2004; accepted 8 February 2005. This work was supported by the BMBF-Roux Research Program of the Martin-Luther-University Halle-Wittenberg, grant number FKZ 2/21, and the Deutsche Krebshilfe, grant number FKZ 70-2787-Bu3. Address correspondence to Friederike Meyer-Wentrup, MD, Children’s Cancer Research Center/Biocenter and Department of Pediatrics, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany. E-mail: friederike.meyer-wentrup@medizin.uni-halle.de

PY - 2005/6

Y1 - 2005/6

N2 - Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFNγ video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.

AB - Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFNγ video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.

KW - Dendritic cells

KW - Ewing family tumors

KW - Peptide epitope

KW - Tumor vaccination

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SN - 0888-0018

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JO - Pediatric Hematology and Oncology

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Identification of an immunogenic EWS-FLI1-derived HLA-DR-restricted T helper cell epitope

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