Identification of MAGE-3 epitopes presented by HLA-DR molecules to CD4+ T lymphocytes

Pascal Chaux, Valérie Vantomme, Vincent Stroobant, Kris Thielemans, Jurgen Corthals, Rosalie Luiten, Alexander M.M. Eggermont, Thierry Boon, Pierre Van Der Bruggen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

218 Citaten (Scopus)

Samenvatting

MAGE-type genes are expressed by many tumors of different histological types and not by normal cells, except for male germline cells, which do not express major histocompatibility complex (MHC) molecules. Therefore, the antigens encoded by MAGE-type genes are strictly tumor specific and common to many tumors. We describe here the identification of the first MAGE-encoded epitopes presented by histocompatibility leukocyte antigen (HLA) class II molecules to CD4+ T lymphocytes. Monocyte-derived dendritic cells were loaded with a MAGE-3 recombinant protein and used to stimulate autologous CD4+ T cells. We isolated CD4+ T cell clones that recognized two different MAGE-3 epitopes, MAGE-3(114-127) and MAGE-3(121-134), both presented by the HLA-DR13 molecule, which is expressed in 20% of Caucasians. The second epitope is also encoded by MAGE-1, -2, and -6. Our procedure should be applicable to other proteins for the identification of new tumor-specific antigens presented by HLA class II molecules. The knowledge of such antigens will be useful for evaluation of the immune response of cancer patients immunized with proteins or with recombinant viruses carrying entire genes coding for tumor antigens. The use of antigenic peptides presented by class II in addition to peptides presented by class I may also improve the efficacy of therapeutic antitumor vaccination.

Originele taal-2Engels
Pagina's (van-tot)767-777
Aantal pagina's11
TijdschriftJournal of Experimental Medicine
Volume189
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 1 mrt. 1999
Extern gepubliceerdJa

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