Imaging windows for long-term intravital imaging: General overview and technical insights

Maria Alieva, Laila Ritsma, Randy J. Giedt, Ralph Weissleder, Jacco Van Rheenen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

130 Citaten (Scopus)

Samenvatting

Intravital microscopy is increasingly used to visualize and quantitate dynamic biological processes at the (sub)cellular level in live animals. By visualizing tissues through imaging windows, individual cells (e.g., cancer, host, or stem cells) can be tracked and studied over a time-span of days to months. Several imaging windows have been developed to access tissues including the brain, superficial fascia, mammary glands, liver, kidney, pancreas, and small intestine among others. Here, we review the development of imaging windows and compare the most commonly used long-term imaging windows for cancer biology: the cranial imaging window, the dorsal skin fold chamber, the mammary imaging window, and the abdominal imaging window. Moreover, we provide technical details, considerations, and trouble-shooting tips on the surgical procedures and microscopy setups for each imaging window and explain different strategies to assure imaging of the same area over multiple imaging sessions. This review aims to be a useful resource for establishing the long-term intravital imaging procedure.

Originele taal-2Engels
Artikelnummere29917
TijdschriftIntraVital
Volume3
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 2014
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Imaging windows for long-term intravital imaging: General overview and technical insights'. Samen vormen ze een unieke vingerafdruk.

Citeer dit