TY - JOUR
T1 - Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples
AU - Hubeek, I.
AU - Giovannetti, E.
AU - Broekhuizen, A. J.F.
AU - Pastor-Anglada, M.
AU - Kaspers, G. J.L.
AU - Peters, G. J.
PY - 2008/6
Y1 - 2008/6
N2 - Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.
AB - Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.
KW - Antimetabolite cytotoxic drugs
KW - Deoxynucleoside analogs
KW - Human concentrative nucleoside transporters (hCNTs)
KW - Human equilibrative nucleoside transporters (hENTs)
KW - Immunocytochemical staining
UR - http://www.scopus.com/inward/record.url?scp=46749084205&partnerID=8YFLogxK
U2 - 10.1080/15257770802145942
DO - 10.1080/15257770802145942
M3 - Article
C2 - 18600541
AN - SCOPUS:46749084205
SN - 1525-7770
VL - 27
SP - 787
EP - 793
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 6-7
ER -