Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

I. Hubeek; E. Giovannetti; A. J.F. Broekhuizen; M. Pastor-Anglada; G. J.L. Kaspers; G. J. Peters

doi:10.1080/15257770802145942

Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

I. Hubeek, E. Giovannetti, A. J.F. Broekhuizen, M. Pastor-Anglada, G. J.L. Kaspers, G. J. Peters

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

8 Citaten (Scopus)

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Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.

Originele taal-2	Engels
Pagina's (van-tot)	787-793
Aantal pagina's	7
Tijdschrift	Nucleosides, Nucleotides and Nucleic Acids
Volume	27
Nummer van het tijdschrift	6-7
DOI's	https://doi.org/10.1080/15257770802145942
Status	Gepubliceerd - jun. 2008
Extern gepubliceerd	Ja

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title = "Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples",

abstract = "Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.",

keywords = "Antimetabolite cytotoxic drugs, Deoxynucleoside analogs, Human concentrative nucleoside transporters (hCNTs), Human equilibrative nucleoside transporters (hENTs), Immunocytochemical staining",

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AU - Giovannetti, E.

AU - Broekhuizen, A. J.F.

AU - Pastor-Anglada, M.

AU - Kaspers, G. J.L.

AU - Peters, G. J.

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AB - Nucleoside transporters are essential for the cellular entry, efficacy, and cytotoxicity of several clinically important deoxynucleoside analogs (e.g., cytarabine and gemcitabine). We used immunohistochemistry to determine protein expression levels of the nucleoside transporters hENT1 and hCNT1 in NSCLC cell lines, NSCLC patient samples, and a variety of normal tissues. All 4 NSCLC cell lines expressed high to very high levels of both hENT1 and hCNT1. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. Immunohistochemistry might be a useful tool to predict response to deoxynucleoside analogs in malignancies treated with these drugs.

KW - Antimetabolite cytotoxic drugs

KW - Deoxynucleoside analogs

KW - Human concentrative nucleoside transporters (hCNTs)

KW - Human equilibrative nucleoside transporters (hENTs)

KW - Immunocytochemical staining

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Immunocytochemical detection of hENT1 and hCNT1 in normal tissues, lung cancer cell lines, and NSCLC patient samples

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