Immunosurveillance against tetraploidization-induced colon tumorigenesis

Alice Boilève, Laura Senovilla, Ilio Vitale, Delphine Lissa, Isabelle Martins, Didier Métivier, Stieneke Van Den Brink, Hans Clevers, Lorenzo Galluzzi, Maria Castedo, Guido Kroemer

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

35 Citaten (Scopus)

Samenvatting

Circumstantial evidence suggests that colon carcinogenesis can ensue the transient tetraploidization of (pre-)malignant cells. In line with this notion, the tumor suppressors APC and TP 53, both of which are frequently inactivated in colon cancer, inhibit tetraploidization in vitro and in vivo. Here, we show that - contrarily to their wild-type counterparts - Tp53-/- colonocytes are susceptible to drug-induced or spontaneous tetraploidization in vitro. Colon organoids generated from tetraploid Tp53-/- cells exhibit a close-to-normal morphology as compared to their diploid Tp53 -/- counterparts, yet the colonocytes constituting these organoids are characterized by an increased cell size and an elevated expression of the immunostimulatory protein calreticulin on the cell surface. The subcutaneous injection of tetraploid Tp53-/- colon organoids led to the generation of proliferating tumors in immunodeficient, but not immunocompetent, mice. Thus, tetraploid Tp53-/- colonocytes fail to survive in immunocompetent mice and develop neoplastic lesions in immunocompromised settings only. These results suggest that tetraploidy is particularly oncogenic in the context of deficient immunosurveillance.

Originele taal-2Engels
Pagina's (van-tot)473-479
Aantal pagina's7
TijdschriftCell Cycle
Volume12
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 1 feb. 2013
Extern gepubliceerdJa

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