In Childhood Acute Lymphoblastic Leukemia, Blasts at Different Stages of Immunophenotypic Maturation Have Stem Cell Properties

Christoph le Viseur, Marc Hotfilder, Simon Bomken, Kerrie Wilson, Silja Röttgers, André Schrauder, Annegret Rosemann, Julie Irving, Ronald W. Stam, Leonard D. Shultz, Jochen Harbott, Heribert Jürgens, Martin Schrappe, Rob Pieters, Josef Vormoor

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

219 Citaten (Scopus)

Samenvatting

We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.

Originele taal-2Engels
Pagina's (van-tot)47-58
Aantal pagina's12
TijdschriftCancer Cell
Volume14
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 8 jul. 2008
Extern gepubliceerdJa

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