TY - JOUR
T1 - In Situ Detection of HY-Specific T Cells in Acute Graft-versus-Host Disease-Affected Male Skin after Sex-Mismatched Stem Cell Transplantation
AU - Kim, Yeung Hyen
AU - Faaij, Claudia M.J.M.
AU - van Halteren, Astrid G.S.
AU - Schrama, Ellen
AU - de Jong, Trees A.M.
AU - Schøller, Jørgen
AU - Egeler, R. Maarten
AU - Pavel, Stan
AU - Vyth-Dreese, Florry A.
AU - van Tol, Maarten J.D.
AU - Goulmy, Els
AU - Spierings, Eric
N1 - Funding Information:
Financial disclosure: This study was funded in part by Dutch Cancer Society Grants UL 2003-2804 (to Yeung-Hyen Kim, Ellen Schrama, and Els Goulmy) and UL 2005-3657 (to Claudia M. J. M. Faaij and Astrid G. S. van Halteren) and by the Netherlands Organization for Scientific Research/NWO Spinoza Award (to Els Goulmy). Eric Spierings is a former special fellow of the Leukemia and Lymphoma Society of America (3314-05). Jørgen Schøller is employed as Chief Scientific Officer at Immudex. The other authors have no conflicts of interest to disclose.
PY - 2012/3
Y1 - 2012/3
N2 - HY-specific T cells are presumed to play a role in acute graft-versus-host disease (aGVHD) after female-to-male stem cell transplantation (SCT). However, infiltrates of these T cells in aGVHD-affected tissues have not yet been reported. We evaluated the application of HLA-A2/HY dextramers for the in situ detection of HY-specific T cells in cryopreserved skin biopsy specimens. We applied the HLA-A2/HY dextramers on cryopreserved skin biopsy specimens from seven male HLA-A2+ pediatric patients who underwent stem cell transplantation with confirmed aGVHD involving the skin. The dextramers demonstrated the presence of HY-specific T cells. In skin biopsy specimens of three male recipients of female grafts, 68% to 78% of all skin-infiltrating CD8+ T cells were HY-specific, whereas these cells were absent in biopsy specimens collected from sex-matched patient-donor pairs. Although this study involved a small and heterogeneous patient group, our results strongly support the hypothesis that HY-specific T cells are actively involved in the pathophysiology of aGVHD after sex-mismatched stem cell transplantation.
AB - HY-specific T cells are presumed to play a role in acute graft-versus-host disease (aGVHD) after female-to-male stem cell transplantation (SCT). However, infiltrates of these T cells in aGVHD-affected tissues have not yet been reported. We evaluated the application of HLA-A2/HY dextramers for the in situ detection of HY-specific T cells in cryopreserved skin biopsy specimens. We applied the HLA-A2/HY dextramers on cryopreserved skin biopsy specimens from seven male HLA-A2+ pediatric patients who underwent stem cell transplantation with confirmed aGVHD involving the skin. The dextramers demonstrated the presence of HY-specific T cells. In skin biopsy specimens of three male recipients of female grafts, 68% to 78% of all skin-infiltrating CD8+ T cells were HY-specific, whereas these cells were absent in biopsy specimens collected from sex-matched patient-donor pairs. Although this study involved a small and heterogeneous patient group, our results strongly support the hypothesis that HY-specific T cells are actively involved in the pathophysiology of aGVHD after sex-mismatched stem cell transplantation.
KW - Antigen/peptide/epitopes
KW - Hematopoietic stem cell transplantation
KW - Human
KW - Minor histocompatibility antigen
KW - Pediatric
UR - https://www.scopus.com/pages/publications/84856966499
U2 - 10.1016/j.bbmt.2011.10.038
DO - 10.1016/j.bbmt.2011.10.038
M3 - Article
C2 - 22062805
AN - SCOPUS:84856966499
SN - 1083-8791
VL - 18
SP - 381
EP - 387
JO - Biology of Blood and Marrow Transplantation
JF - Biology of Blood and Marrow Transplantation
IS - 3
ER -