In situ hybridization to identify gut stem cells

Alex Gregorieff; Hans Clevers

doi:10.1002/9780470151808.sc02f01s34

In situ hybridization to identify gut stem cells

Alex Gregorieff, Hans Clevers

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

27 Citaten (Scopus)

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In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

Originele taal-2	Engels
Pagina's (van-tot)	2F.1.1-2F.1.10
Tijdschrift	Current Protocols in Stem Cell Biology
Volume	2015
DOI's	https://doi.org/10.1002/9780470151808.sc02f01s34 https://doi.org/10.1002/9780470151808.sc02f01s12
Status	Gepubliceerd - 2015
Extern gepubliceerd	Ja

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title = "In situ hybridization to identify gut stem cells",

abstract = "In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.",

keywords = "(ISH) in situ hybridization, Digoxigenin RNA probes, Formalin-fixed paraffin-embedded, Intestinal sections",

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AU - Clevers, Hans

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KW - (ISH) in situ hybridization

KW - Digoxigenin RNA probes

KW - Formalin-fixed paraffin-embedded

KW - Intestinal sections

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In situ hybridization to identify gut stem cells

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