TY - JOUR
T1 - In vivo bioluminescence imaging of cell differentiation in biomaterials
T2 - A platform for scaffold development
AU - Bagó, Juli R.
AU - Aguilar, Elisabeth
AU - Alieva, Maria
AU - Soler-Botija, Carolina
AU - Vila, Olaia F.
AU - Claros, Silvia
AU - Andrades, José A.
AU - Becerra, José
AU - Rubio, Nuria
AU - Blanco, Jerónimo
PY - 2013/3/1
Y1 - 2013/3/1
N2 - In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior in biomaterials implanted in live animals should facilitate the development of scaffolds for tissue engineering. We report on a noninvasive bioluminescence imaging (BLI) procedure for simultaneous monitoring of changes in the expression of multiple genes to evaluate scaffold performance in vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly green fluorescent protein chimeric reporters regulated by cytomegalovirus and tissue-specific promoters, respectively. Labeled cells were induced to differentiate in vitro and in vivo, by seeding in demineralized bone matrices (DBMs) and monitored by BLI. Imaging results were validated by RT-polymerase chain reaction and histological procedures. The proposed approach improves molecular imaging and measurement of changes in gene expression of cells implanted in live animals. This procedure, applicable to the simultaneous analysis of multiple genes from cells seeded in DBMs, should facilitate engineering of scaffolds for tissue repair.
AB - In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior in biomaterials implanted in live animals should facilitate the development of scaffolds for tissue engineering. We report on a noninvasive bioluminescence imaging (BLI) procedure for simultaneous monitoring of changes in the expression of multiple genes to evaluate scaffold performance in vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly green fluorescent protein chimeric reporters regulated by cytomegalovirus and tissue-specific promoters, respectively. Labeled cells were induced to differentiate in vitro and in vivo, by seeding in demineralized bone matrices (DBMs) and monitored by BLI. Imaging results were validated by RT-polymerase chain reaction and histological procedures. The proposed approach improves molecular imaging and measurement of changes in gene expression of cells implanted in live animals. This procedure, applicable to the simultaneous analysis of multiple genes from cells seeded in DBMs, should facilitate engineering of scaffolds for tissue repair.
UR - http://www.scopus.com/inward/record.url?scp=84873368335&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2012.0073
DO - 10.1089/ten.tea.2012.0073
M3 - Article
C2 - 23013334
AN - SCOPUS:84873368335
SN - 1937-3341
VL - 19
SP - 593
EP - 603
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 5-6
ER -