Insulin stimulation of gene expression mediated by p21ras activation

B. M.T. Burgering, R. H. Medema, J. A. Maassen, M. L. Van De Wetering, A. Van Der Eb, F. McCormick, J. L. Bos

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

279 Citaten (Scopus)


In fibroblasts, insulin is a weak mitogen and does not induce expression of c-fos, c-jun or p33. However, increasing the expression levels of either normal p21Hras or the insulin receptor, but not mutant p21Hras, enables insulin to induce the expression of these genes. In cells expressing elevated levels of insulin receptor, this process involves a rapid increase in p21rasGTP levels (from 20% to 70% GTP as a percentage of total guanine nucleotides). No increase in p2lrasGTP levels was observed after PDGF and EGF stimulation of cells expressing high levels of the cognate receptor, stressing the specificity of the insulin-induced increase. We conclude that in fibroblasts, p2lras is an intermediate of the insulin signal transduction pathway involved in the regulation of gene expression and mitogenicity.

Originele taal-2Engels
Pagina's (van-tot)1103-1109
Aantal pagina's7
TijdschriftEMBO Journal
Nummer van het tijdschrift5
StatusGepubliceerd - 1991
Extern gepubliceerdJa


Duik in de onderzoeksthema's van 'Insulin stimulation of gene expression mediated by p21ras activation'. Samen vormen ze een unieke vingerafdruk.

Citeer dit