Intraclonal Plasticity in Mammary Tumors Revealed through Large-Scale Single-Cell Resolution 3D Imaging

3DTCLSM Group

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

107 Citaten (Scopus)

Samenvatting

Breast tumors are inherently heterogeneous, but the evolving cellular organization through neoplastic progression is poorly understood. Here we report a rapid, large-scale single-cell resolution 3D imaging protocol based on a one-step clearing agent that allows visualization of normal tissue architecture and entire tumors at cellular resolution. Imaging of multicolor lineage-tracing models of breast cancer targeted to either basal or luminal progenitor cells revealed profound clonal restriction during progression. Expression profiling of clones arising in Pten/Trp53-deficient tumors identified distinct molecular signatures. Strikingly, most clones harbored cells that had undergone an epithelial-to-mesenchymal transition, indicating widespread, inherent plasticity. Hence, an integrative pipeline that combines lineage tracing, 3D imaging, and clonal RNA sequencing technologies offers a comprehensive path for studying mechanisms underlying heterogeneity in whole tumors.

Originele taal-2Engels
Pagina's (van-tot)618-632.e6
TijdschriftCancer Cell
Volume35
Nummer van het tijdschrift4
DOI's
StatusGepubliceerd - 15 apr. 2019

Vingerafdruk

Duik in de onderzoeksthema's van 'Intraclonal Plasticity in Mammary Tumors Revealed through Large-Scale Single-Cell Resolution 3D Imaging'. Samen vormen ze een unieke vingerafdruk.

Citeer dit