@article{e3c77f260e164e3c8ca74329ac9f1c0b,
title = "Isolation and in vitro expansion of human colonic stem cells",
abstract = "Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.",
author = "Peter Jung and Toshiro Sato and Anna Merlos-Su{\'a}rez and Barriga, {Francisco M.} and Mar Iglesias and David Rossell and Herbert Auer and Mercedes Gallardo and Blasco, {Maria A.} and Elena Sancho and Hans Clevers and Eduard Batlle",
note = "Funding Information: We thank Genentech for the EphB2-specific antibody Mab 2H9 and the Institute for Research in Biomedicine transcriptomic facility, electronic microscopy service (Universitat de Barcelona) and Cell separation unit (UB) for expert assistance. We thank L. Lopez Vilaro and E. Calder{\'o}n G{\'o}mez for help with mucosas. This work is supported by grants to E.B. from the CONSOLIDER and Plan Nacional I+D+i Programs from Spanish Ministry of Science and Innovation (MICINN) and the European Research Council (ERC-FP7). Work was furthermore supported by grants from Instituto de Salud Carlos III FEDER (RD09/0076/00036). The {\textquoteleft}Xarxa de Bancs de tumors{\textquoteright} is sponsored by Pla Director d{\textquoteright}Oncologia de Catalunya (XBTC). The M.A.B. laboratory is founded by the MICINN, the EU, the Botin and Lilly Foundations, and the Korber European Science Award to M.A.B. P.J. holds a Marie Curie Fellowship (FP7).",
year = "2011",
month = oct,
doi = "10.1038/nm.2470",
language = "English",
volume = "17",
pages = "1225--1227",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "10",
}