Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

J. H.J. Hoeijmakers; P. J. Welters; G. J. Brakenhoff; P. Borst

doi:10.1016/0147-619X(82)90003-8

Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

J. H.J. Hoeijmakers, P. J. Welters, G. J. Brakenhoff, P. Borst

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

11 Citaten (Scopus)

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We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

Originele taal-2	Engels
Pagina's (van-tot)	221-229
Aantal pagina's	9
Tijdschrift	Plasmid
Volume	7
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1016/0147-619X(82)90003-8
Status	Gepubliceerd - mei 1982
Extern gepubliceerd	Ja

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10.1016/0147-619X(82)90003-8

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title = "Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles",

abstract = "We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex {"}eyes{"} interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of {"}eyed{"} molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also {"}eyes{"} were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.",

author = "Hoeijmakers, {J. H.J.} and Welters, {P. J.} and Brakenhoff, {G. J.} and P. Borst",

note = "Funding Information: We are indebtedt o the Staff of the Laboratory of Electron Microscopy( Head Prof. N. Nanninga) of the University of Amsterdamf or their generousp rovision of facilities.W e thank Miss J. Van den Burg and Miss M. Van den Berg for expert technicala ssistanceM, rs. Rita Boucke for typing of the manuscript,a nd Mr. T. Van OS and Mr. J. Woonsf or helpw ith the photographs. This work was supportedi n part by a grant from The Netherlands Foundation for Fundamental Biological Research( BION) with financial aid from The Orga-nizationf or the Advancemenot f Pure Research( ZWO).",

year = "1982",

month = may,

doi = "10.1016/0147-619X(82)90003-8",

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journal = "Plasmid",

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T1 - Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

AU - Hoeijmakers, J. H.J.

AU - Welters, P. J.

AU - Brakenhoff, G. J.

AU - Borst, P.

N1 - Funding Information: We are indebtedt o the Staff of the Laboratory of Electron Microscopy( Head Prof. N. Nanninga) of the University of Amsterdamf or their generousp rovision of facilities.W e thank Miss J. Van den Burg and Miss M. Van den Berg for expert technicala ssistanceM, rs. Rita Boucke for typing of the manuscript,a nd Mr. T. Van OS and Mr. J. Woonsf or helpw ith the photographs. This work was supportedi n part by a grant from The Netherlands Foundation for Fundamental Biological Research( BION) with financial aid from The Orga-nizationf or the Advancemenot f Pure Research( ZWO).

PY - 1982/5

Y1 - 1982/5

N2 - We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

AB - We have investigated the nature of the sequence heterogeneity of the minicircles of Crithidia luciliae kinetoplast DNA by EM heteroduplex analysis of minicircles cleaved with endonuclease HindIII. Approximately 40% of the minicircles showed-after denaturation and reannealing-structures indicative of sequence rearrangements: the majority contained heteroduplex "eyes" interpreted as due to inversions; about 10% of the heteroduplexes yielded structures interpreted as due to translocations and a similar fraction showed insertions and deletions. The category of "eyed" molecules was analyzed in detail: four minicircle segments were found that displayed a high incidence of such eyes indicating that the rearrangements were not scattered at random over the minicircles. Moreover, since also "eyes" were found overlapping two or three of the four regions, we postulate that these segments are capable of recombining with each other. We conclude that specific segmental rearrangements form the main basis of the minicircle sequence heterogeneity in Crithidia.

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Kinetoplast DNA in the insect trypanosomes Crithidia luciliae and Crithidia fasciculata. III. Heteroduplex analysis of the C. luciliae minicircles

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