Lineage tracing of mammary stem and progenitor cells

Anoeska A.A. van de Moosdijk, Nai Yang Fu, Anne C. Rios, Jane E. Visvader, Renée van Amerongen

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukpeer review

8 Citaten (Scopus)

Samenvatting

Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (CreERT2/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.

Originele taal-2Engels
TitelMethods in Molecular Biology
UitgeverijHumana Press Inc.
Pagina's291-308
Aantal pagina's18
DOI's
StatusGepubliceerd - 2017
Extern gepubliceerdJa

Publicatie series

NaamMethods in Molecular Biology
Volume1501
ISSN van geprinte versie1064-3745

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