TY - JOUR
T1 - Local interferon—γ levels during respiratory syncytial virus lower respiratory tract infection are associated with disease severity
AU - Bont, Louis
AU - Heijnen, Cobi J.
AU - Kavelaars, Annemieke
AU - Van Aalderen, Wim M.C.
AU - Brus, Frank
AU - Draaisma, Jos M.Th
AU - Pekelharing-Berghuis, Martha
AU - Van Diemen-Steenvoorde, Ronnie A.A.M.
AU - Kimpen, Jan L.L.
N1 - Funding Information:
Received 4 January 2001; revised 21 March 2001; electronically published 26 June 2001. The study was approved by the medical ethical committees at all participating centers. Parents of subjects gave written informed consent. Financial support: Dutch Asthma Foundation (grant 32.96.08). Reprints or correspondence: Dr. Jan L. L. Kimpen, Dept. of Pediatric Infectious Diseases, Wilhelmina Children’s Hospital, University Medical Center, PO Box 85500, 3508 GA Utrecht, The Netherlands (j.kimpen@ wkz.azu.nl).
PY - 2001/8/1
Y1 - 2001/8/1
N2 - To investigate the role of cell-mediated immunity during respiratory syncytial virus (RSV) infection, interferon (IFN)-γ and interleukin (IL)-10 levels in nasopharyngeal secretions were measured in infants with lower respiratory tract infection (LRTI) caused by RSV. A novel technique was used to measure in vivo cytokine levels in nasopharyngeal aspirates (NPAs). Cytokine levels in the NPAs of 17 mechanically ventilated infants and 43 nonventilated hospitalized infants were compared. As expected, mechanically ventilated infants were significantly younger than nonventilated infants (7 vs. 14 weeks). IFN-γ levels were above the limit of detection in the NPAs of 3 (18%) mechanically ventilated infants and in the NPAs of 26 (60%) nonventilated infants. IL-10 levels in the NPAs of mechanically ventilated and nonventilated infants were comparable. It is hypothesized that maturation-related mechanisms have a key role in the development of RSV LRTI that results in mechanical ventilation.
AB - To investigate the role of cell-mediated immunity during respiratory syncytial virus (RSV) infection, interferon (IFN)-γ and interleukin (IL)-10 levels in nasopharyngeal secretions were measured in infants with lower respiratory tract infection (LRTI) caused by RSV. A novel technique was used to measure in vivo cytokine levels in nasopharyngeal aspirates (NPAs). Cytokine levels in the NPAs of 17 mechanically ventilated infants and 43 nonventilated hospitalized infants were compared. As expected, mechanically ventilated infants were significantly younger than nonventilated infants (7 vs. 14 weeks). IFN-γ levels were above the limit of detection in the NPAs of 3 (18%) mechanically ventilated infants and in the NPAs of 26 (60%) nonventilated infants. IL-10 levels in the NPAs of mechanically ventilated and nonventilated infants were comparable. It is hypothesized that maturation-related mechanisms have a key role in the development of RSV LRTI that results in mechanical ventilation.
UR - http://www.scopus.com/inward/record.url?scp=0035425846&partnerID=8YFLogxK
U2 - 10.1086/322035
DO - 10.1086/322035
M3 - Article
C2 - 11443563
AN - SCOPUS:0035425846
SN - 1537-6613
VL - 184
SP - 355
EP - 358
JO - The Journal of infectious diseases
JF - The Journal of infectious diseases
IS - 3
ER -