Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence

L H Looijenga, V T Smit, J W Wessels, P Mollevanger, J W Oosterhuis, C J Cornelisse, P Devilee

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.

Originele taal-2Engels
Pagina's (van-tot)216-8
Aantal pagina's3
TijdschriftCytogenetics and cell genetics
Volume53
Nummer van het tijdschrift4
DOI's
StatusGepubliceerd - 1990
Extern gepubliceerdJa

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