Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31

M. H.M. Koken; E. M.E. Smit; I. Jaspers-Dekker; B. A. Oostra; A. Hagemeuer; D. Bootsma; J. H.J. Hoeumakers

doi:10.1016/0888-7543(92)90433-S

Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31

M. H.M. Koken, E. M.E. Smit, I. Jaspers-Dekker, B. A. Oostra, A. Hagemeuer, D. Bootsma, J. H.J. Hoeumakers

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The chromosomal localizations of two closely related human DNA repair genes, HHR6A and HHR6B, were determined by in situ hybridization with biotinylated probes. HHR6A and HHR6B (human homolog of yeast RAD6) encode ubiquitin-conjugating enzymes (E2 enzymes), likely to be involved in postreplication repair and induced mutagenesis. The HHR6B gene was assigned to human chromosome 5q23-q31, whereas the HHR6A gene was localized on the human X chromosome (Xq24-q25). This latter assignment was confirmed with an X-specific human-mouse/hamster somatic cell hybrid panel. Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse. The potential involvement of these genes in human genetic disorders is discussed.

Originele taal-2	Engels
Pagina's (van-tot)	447-453
Aantal pagina's	7
Tijdschrift	Genomics
Volume	12
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1016/0888-7543(92)90433-S
Status	Gepubliceerd - mrt. 1992
Extern gepubliceerd	Ja

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@article{7a764a784ec742ea8f4e247ed069575e,

title = "Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31",

abstract = "The chromosomal localizations of two closely related human DNA repair genes, HHR6A and HHR6B, were determined by in situ hybridization with biotinylated probes. HHR6A and HHR6B (human homolog of yeast RAD6) encode ubiquitin-conjugating enzymes (E2 enzymes), likely to be involved in postreplication repair and induced mutagenesis. The HHR6B gene was assigned to human chromosome 5q23-q31, whereas the HHR6A gene was localized on the human X chromosome (Xq24-q25). This latter assignment was confirmed with an X-specific human-mouse/hamster somatic cell hybrid panel. Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse. The potential involvement of these genes in human genetic disorders is discussed.",

author = "Koken, {M. H.M.} and Smit, {E. M.E.} and I. Jaspers-Dekker and Oostra, {B. A.} and A. Hagemeuer and D. Bootsma and Hoeumakers, {J. H.J.}",

note = "Funding Information: We are indebted to Drs. D. Nelson (Houston) and B. Wieringa (Nijmegen) for providing us with the DNAs from the rodent/human X-chromosome-specific hybrids; Dr. H. Willard (Stanford) for providing us with the pBamX5 probe; Mirko Kuit and Tom de Vries Lentsch for photography; and Drs. A. Geurts van Kessel (Nijmegen) and J. den Dunnen (Leiden) for initial efforts to map these two genes. This work was supported by the Dutch Cancer Society (Project IKR 88-02 and 90-20) and European Community Contract B16-141-NL.",

year = "1992",

month = mar,

doi = "10.1016/0888-7543(92)90433-S",

language = "English",

volume = "12",

pages = "447--453",

journal = "Genomics",

issn = "0888-7543",

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T1 - Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31

AU - Koken, M. H.M.

AU - Smit, E. M.E.

AU - Jaspers-Dekker, I.

AU - Oostra, B. A.

AU - Hagemeuer, A.

AU - Bootsma, D.

AU - Hoeumakers, J. H.J.

N1 - Funding Information: We are indebted to Drs. D. Nelson (Houston) and B. Wieringa (Nijmegen) for providing us with the DNAs from the rodent/human X-chromosome-specific hybrids; Dr. H. Willard (Stanford) for providing us with the pBamX5 probe; Mirko Kuit and Tom de Vries Lentsch for photography; and Drs. A. Geurts van Kessel (Nijmegen) and J. den Dunnen (Leiden) for initial efforts to map these two genes. This work was supported by the Dutch Cancer Society (Project IKR 88-02 and 90-20) and European Community Contract B16-141-NL.

PY - 1992/3

Y1 - 1992/3

N2 - The chromosomal localizations of two closely related human DNA repair genes, HHR6A and HHR6B, were determined by in situ hybridization with biotinylated probes. HHR6A and HHR6B (human homolog of yeast RAD6) encode ubiquitin-conjugating enzymes (E2 enzymes), likely to be involved in postreplication repair and induced mutagenesis. The HHR6B gene was assigned to human chromosome 5q23-q31, whereas the HHR6A gene was localized on the human X chromosome (Xq24-q25). This latter assignment was confirmed with an X-specific human-mouse/hamster somatic cell hybrid panel. Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse. The potential involvement of these genes in human genetic disorders is discussed.

AB - The chromosomal localizations of two closely related human DNA repair genes, HHR6A and HHR6B, were determined by in situ hybridization with biotinylated probes. HHR6A and HHR6B (human homolog of yeast RAD6) encode ubiquitin-conjugating enzymes (E2 enzymes), likely to be involved in postreplication repair and induced mutagenesis. The HHR6B gene was assigned to human chromosome 5q23-q31, whereas the HHR6A gene was localized on the human X chromosome (Xq24-q25). This latter assignment was confirmed with an X-specific human-mouse/hamster somatic cell hybrid panel. Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse. The potential involvement of these genes in human genetic disorders is discussed.

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Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31

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