Long-Term Expansion of Murine Primary Hepatocyte Organoids

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukpeer review


The in vitro maintenance and expansion of primary hepatocytes provide immense opportunities for disease modeling and other biological, viral, and toxicological studies, as well as for applications in regenerative medicine such as cell transplantation for the treatment of metabolic liver diseases. Here, we describe a protocol for the isolation and in vitro culture of primary hepatocytes in a three-dimensional extracellular matrix gel. The inflammatory cytokine tumor necrosis factor alpha (TNFα) is crucial for the long-term expansion of mouse hepatocyte organoids by mimicking the regenerative response in vitro. Long-term cultured hepatocyte organoids express high levels of hepatocyte markers upon differentiation.

Originele taal-2Engels
TitelMethods in Molecular Biology
UitgeverijHumana Press Inc.
Aantal pagina's13
StatusGepubliceerd - 2022

Publicatie series

NaamMethods in molecular biology (Clifton, N.J.)
UitgeverijHumana Press
ISSN van geprinte versie1064-3745


  • Culture
  • Cytokines
  • Hepatocytes
  • Liver
  • Mouse
  • Organoids
  • Regeneration
  • TNFα


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