Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma

Bruce Elliott, Richard A. Scolyer, Stefan Suciu, Serge Lebecque, Donata Rimoldi, Oliver Gugerli, Elena Musat, Raghwa N. Sharma, Danielle Lienard, Ulrich Keilholz, Alessandro Testori, Alexander Eggermont, Rona MacKie, Caroline Robert, Martin Cook, John F. Thompson, Eric Angevin, Alain Spatz

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62 Citaten (Scopus)


Purpose: In a previous immunohistochemical study of dendritic cells (DC) in sentinel lymph nodes (SLN) draining regressing melanomas, we found that the accumulation of mature DC-LAMP+ DCs in SLNs was associated with local expansion of antigen-specific memory effector CTLs and the absence of metastasis in downstream lymph nodes. The aim of this study was to investigate the prognostic importance of the maximal density of mature DCs in SLNs. Experimental Design: A total of 458 consecutive patients with micrometastatic melanoma within SLNs were eligible for analysis. The maximal density of mature DC-LAMP+ DCs was evaluated by three independent observers and categorized into three classes (<100, 100 to <200, and ≥200/mm 2). Results: There was excellent interobserver reproducibility for maximum density of mature DC-LAMP+ DC scores (κ score = 0.82). There were differences in the maximal density scores and staining intensity according to the treating melanoma center (P < 0.001). The higher the mature DC density in the SLNis, the longer is the duration of survival [P = 0.047; hazard ratio, 0.70; 95% confidence interval, 0.50-1.00]. Adjusted by thickness and ulceration, the prognostic importance of DC density was lower (P = 0.36). Conclusion: This study is the first to report the prognostic value of DC-LAMP+ DC counts in SLNs containing metastatic melanoma. Patients with a high density of mature DCs (≥200/mm2) have the lowest risk of death. It also provides evidence that a lack of maturation in the SLNs is important in biological facilitation of melanoma progression.

Originele taal-2Engels
Pagina's (van-tot)3825-3830
Aantal pagina's6
TijdschriftClinical Cancer Research
Nummer van het tijdschrift13
StatusGepubliceerd - 1 jul. 2007
Extern gepubliceerdJa


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