Samenvatting
Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accumulation of β-catenin. Coincidentally, it perturbs differentiation, migration, proliferation, and apoptosis, such that Apc-deficient cells maintain a "crypt progenitor-like" phenotype. Critically, for the first time we confirm a series of Wnt target molecules in an in vivo setting and also identify a series of new candidate targets within the same setting.
Originele taal-2 | Engels |
---|---|
Pagina's (van-tot) | 1385-1390 |
Aantal pagina's | 6 |
Tijdschrift | Genes and Development |
Volume | 18 |
Nummer van het tijdschrift | 12 |
DOI's | |
Status | Gepubliceerd - 15 jun. 2004 |
Extern gepubliceerd | Ja |