Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

Eugene Berezikov; Geert Van Tetering; Mark Verheul; Jose Van De Belt; Linda Van Laake; Joost Vos; Robert Verloop; Marc Van De Wetering; Victor Guryev; Shuji Takada; Anton Jan Van Zonneveld; Hiroyuki Mano; Ronald Plasterk; Edwin Cuppen

doi:10.1101/gr.5159906

Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

Eugene Berezikov, Geert Van Tetering, Mark Verheul, Jose Van De Belt, Linda Van Laake, Joost Vos, Robert Verloop, Marc Van De Wetering, Victor Guryev, Shuji Takada, Anton Jan Van Zonneveld, Hiroyuki Mano, Ronald Plasterk, Edwin Cuppen

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

240 Citaten (Scopus)

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MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ∼10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.

Originele taal-2	Engels
Pagina's (van-tot)	1289-1298
Aantal pagina's	10
Tijdschrift	Genome Research
Volume	16
Nummer van het tijdschrift	10
DOI's	https://doi.org/10.1101/gr.5159906
Status	Gepubliceerd - 2006
Extern gepubliceerd	Ja

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Berezikov, E., Van Tetering, G., Verheul, M., Van De Belt, J., Van Laake, L., Vos, J., Verloop, R., Van De Wetering, M., Guryev, V., Takada, S., Van Zonneveld, A. J., Mano, H., Plasterk, R., & Cuppen, E. (2006). Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Research, 16(10), 1289-1298. https://doi.org/10.1101/gr.5159906

@article{7edd53d1ecbe46ea8b73f38d8821c725,

title = "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis",

abstract = "MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ∼10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.",

author = "Eugene Berezikov and {Van Tetering}, Geert and Mark Verheul and {Van De Belt}, Jose and {Van Laake}, Linda and Joost Vos and Robert Verloop and {Van De Wetering}, Marc and Victor Guryev and Shuji Takada and {Van Zonneveld}, {Anton Jan} and Hiroyuki Mano and Ronald Plasterk and Edwin Cuppen",

year = "2006",

doi = "10.1101/gr.5159906",

language = "English",

volume = "16",

pages = "1289--1298",

journal = "Genome Research",

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publisher = "Cold Spring Harbor Laboratory Press",

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Berezikov, E, Van Tetering, G, Verheul, M, Van De Belt, J, Van Laake, L, Vos, J, Verloop, R, Van De Wetering, M, Guryev, V, Takada, S, Van Zonneveld, AJ, Mano, H, Plasterk, R & Cuppen, E 2006, 'Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis', Genome Research, vol. 16, nr. 10, blz. 1289-1298. https://doi.org/10.1101/gr.5159906

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T1 - Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

AU - Berezikov, Eugene

AU - Van Tetering, Geert

AU - Verheul, Mark

AU - Van De Belt, Jose

AU - Van Laake, Linda

AU - Vos, Joost

AU - Verloop, Robert

AU - Van De Wetering, Marc

AU - Guryev, Victor

AU - Takada, Shuji

AU - Van Zonneveld, Anton Jan

AU - Mano, Hiroyuki

AU - Plasterk, Ronald

AU - Cuppen, Edwin

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AB - MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently >400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ∼10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.

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VL - 16

SP - 1289

EP - 1298

JO - Genome Research

JF - Genome Research

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Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

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