TY - JOUR
T1 - Mapping of constitutional translocation breakpoints in renal cell cancer patients
T2 - identification of KCNIP4 as a candidate gene
AU - Bonne, Anita
AU - Vreede, Lilian
AU - Kuiper, Roland P
AU - Bodmer, Danielle
AU - Jansen, Corine
AU - Eleveld, Marc
AU - van Erp, Femke
AU - Arkesteijn, Ger
AU - Hoogerbrugge, Nicoline
AU - van Ravenswaaij, Conny
AU - Schoenmakers, Eric F P M
AU - Geurts van Kessel, Ad
PY - 2007/11
Y1 - 2007/11
N2 - Our group and others had previously developed a high throughput procedure to map translocation breakpoints using chromosome flow sorting in conjunction with microarray-based comparative genomic hybridization (arrayCGH). Here we applied both conventional positional cloning and integrated arrayCGH procedures to the mapping of constitutional chromosome anomalies in four patients with renal cell cancer (RCC), three with a chromosome 3 translocation, and one with an insertion involving chromosome 3. In one of these patients, who was carrying a t(3;4)(p13;p15), the KCNIP4 gene was found to be disrupted. KCNIP4 belongs to a family of potassium channel-interacting proteins and is highly expressed in normal kidney cells. In addition, KCNIP4 splice variants have specifically been encountered in RCC.
AB - Our group and others had previously developed a high throughput procedure to map translocation breakpoints using chromosome flow sorting in conjunction with microarray-based comparative genomic hybridization (arrayCGH). Here we applied both conventional positional cloning and integrated arrayCGH procedures to the mapping of constitutional chromosome anomalies in four patients with renal cell cancer (RCC), three with a chromosome 3 translocation, and one with an insertion involving chromosome 3. In one of these patients, who was carrying a t(3;4)(p13;p15), the KCNIP4 gene was found to be disrupted. KCNIP4 belongs to a family of potassium channel-interacting proteins and is highly expressed in normal kidney cells. In addition, KCNIP4 splice variants have specifically been encountered in RCC.
KW - Carcinoma, Renal Cell/genetics
KW - Cell Line, Tumor
KW - Chromosome Breakage
KW - Chromosome Mapping
KW - Chromosomes, Human, Pair 3
KW - Chromosomes, Human, Pair 4
KW - Cloning, Molecular
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Kidney Neoplasms/genetics
KW - Kv Channel-Interacting Proteins/genetics
KW - Loss of Heterozygosity
KW - Mutagenesis, Insertional
KW - Translocation, Genetic
U2 - 10.1016/j.cancergencyto.2007.07.005
DO - 10.1016/j.cancergencyto.2007.07.005
M3 - Article
C2 - 17981209
VL - 179
SP - 11
EP - 18
JO - Cancer genetics and cytogenetics
JF - Cancer genetics and cytogenetics
SN - 0165-4608
IS - 1
ER -