TY - JOUR
T1 - Microinjection of Escherichia coli UvrA, B, C and D proteins into fibroblasts of xeroderma pigmentosum complementation groups A and C does not result in restoration of UV-induced unscheduled DNA synthesis
AU - Zwetsloot, J. C.M.
AU - Barbeiro, A. P.
AU - Vermeulen, W.
AU - Arthur, H. M.
AU - Hoeijmakers, J. H.J.
AU - Backendorf, C.
N1 - Funding Information:
The authors wish to thank Prof. P. van de Putte and Prof. D. Bootsma for their constant encouragement and interest and Dr. B. van Zeeland for the generous gift of T4 endo V. We would like to thank J. Lekkerkerk and M. Dibon-de Ruyter for their help in protein purifications and N. van Hoek for the typographic work. This work was supported by the Foundation for Medical Research FUNGO, contract No. 13-23-35, and by the Commission of the European Communities, contract Nos. BIO-E-404-NL and BIO-E-408-NL.
PY - 1986/7
Y1 - 1986/7
N2 - The UV-induced unscheduled DNA synthesis (UDS) in cultured human fibroblasts of repair-deficient xeroderma pigmentosum complementation groups A and C was assayed after injection of identical activities of either Uvr excinuclease (UvrA, B, C and D) from Escherichia coli or endonuclease V from phage T4. Under conditions where the T4 enzyme was able to induce repair synthesis in both XP complementation groups in agreement with earlier observations (de Jonge et al., 1985), no effect of the UvrABCD excinuclease could be observed either when the enzymatic complex was injected into the cytoplasm, or when it was delivered directly into the nucleus. In additon, no effect of the E. coli excinuclease was found on the repair ability of normal repair-proficient human fibroblasts. We conclude that the UvrABCD excinuclease may not work on DNA lesions in human chromatin.
AB - The UV-induced unscheduled DNA synthesis (UDS) in cultured human fibroblasts of repair-deficient xeroderma pigmentosum complementation groups A and C was assayed after injection of identical activities of either Uvr excinuclease (UvrA, B, C and D) from Escherichia coli or endonuclease V from phage T4. Under conditions where the T4 enzyme was able to induce repair synthesis in both XP complementation groups in agreement with earlier observations (de Jonge et al., 1985), no effect of the UvrABCD excinuclease could be observed either when the enzymatic complex was injected into the cytoplasm, or when it was delivered directly into the nucleus. In additon, no effect of the E. coli excinuclease was found on the repair ability of normal repair-proficient human fibroblasts. We conclude that the UvrABCD excinuclease may not work on DNA lesions in human chromatin.
UR - http://www.scopus.com/inward/record.url?scp=0022548533&partnerID=8YFLogxK
U2 - 10.1016/0167-8817(86)90044-1
DO - 10.1016/0167-8817(86)90044-1
M3 - Article
C2 - 3014326
AN - SCOPUS:0022548533
SN - 0167-8817
VL - 166
SP - 89
EP - 98
JO - Mutation Research DNA Repair Reports
JF - Mutation Research DNA Repair Reports
IS - 1
ER -