Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups

A. J.R. de Jonge; W. Vermeulen; W. Keijzer; J. H.J. Hoeijmakers; D. Bootsma

doi:10.1016/0027-5107(85)90106-X

Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups

A. J.R. de Jonge, W. Vermeulen, W. Keijzer, J. H.J. Hoeijmakers, D. Bootsma

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complemenation groups tested.

Originele taal-2	Engels
Pagina's (van-tot)	99-105
Aantal pagina's	7
Tijdschrift	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	150
Nummer van het tijdschrift	1-2
DOI's	https://doi.org/10.1016/0027-5107(85)90106-X
Status	Gepubliceerd - 1985
Extern gepubliceerd	Ja

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de Jonge, A. J. R., Vermeulen, W., Keijzer, W., Hoeijmakers, J. H. J., & Bootsma, D. (1985). Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 150(1-2), 99-105. https://doi.org/10.1016/0027-5107(85)90106-X

@article{c2ee65a1a57048d1a5207f2eb828c1f7,

title = "Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups",

abstract = "The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complemenation groups tested.",

author = "{de Jonge}, {A. J.R.} and W. Vermeulen and W. Keijzer and Hoeijmakers, {J. H.J.} and D. Bootsma",

year = "1985",

doi = "10.1016/0027-5107(85)90106-X",

language = "English",

volume = "150",

pages = "99--105",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

publisher = "Elsevier BV",

number = "1-2",

}

de Jonge, AJR, Vermeulen, W, Keijzer, W, Hoeijmakers, JHJ & Bootsma, D 1985, 'Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 150, nr. 1-2, blz. 99-105. https://doi.org/10.1016/0027-5107(85)90106-X

Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups. / de Jonge, A. J.R.; Vermeulen, W.; Keijzer, W. et al.
In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 150, Nr. 1-2, 1985, blz. 99-105.

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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AU - de Jonge, A. J.R.

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AB - The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complemenation groups tested.

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Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups

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