MO25α/β interact with STRADα/β enhancing their ability to bind, activate and localize LKB1 in the cytoplasm

Jérôme Boudeau, Annette F. Baas, Maria Deak, Nick A. Morrice, Agnieszka Kieloch, Mike Schutkowski, Alan R. Prescott, Hans C. Clevers, Dario R. Alessi

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

371 Citaten (Scopus)

Samenvatting

Mutations in the LKB1 protein kinase result in the inherited Peutz Jeghers cancer syndrome. LKB1 has been implicated in regulating cell proliferation and polarity although little is known about how this enzyme is regulated. We recently showed that LKB1 is activated through its interaction with STRADα, a catalytically deficient pseudokinase. Here we show that endogenous LKB1-STRADα complex is associated with a protein of unknown function, termed MO25α, through the interaction of MO25α with the last three residues of STRADα. MO25α and STRADα anchor LKB1 in the cytoplasm, excluding it from the nucleus. Moreover, MO25α enhances the formation of the LKB1-STRADα complex in vivo, stimulating the catalytic activity of LKB1-10-fold. We demonstrate that the related STRADβ and MO25β isoforms are also able to stabilize LKB1 in an active complex and that it is possible to isolate complexes of LKB1 bound to STRAD and MO25 isoforms, in which the subunits are present in equimolar amounts. Our results indicate that MO25 may function as a scaffolding component of the LKB1-STRAD complex and plays a crucial role in regulating LKB1 activity and cellular localization.

Originele taal-2Engels
Pagina's (van-tot)5102-5114
Aantal pagina's13
TijdschriftEMBO Journal
Volume22
Nummer van het tijdschrift19
DOI's
StatusGepubliceerd - 1 okt. 2003
Extern gepubliceerdJa

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