TY - JOUR
T1 - Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors
AU - Sinke, R. J.
AU - Olde Weghuis, D.
AU - Suijkerbuijk, R. F.
AU - Tanigami, A.
AU - Nakamura, Y.
AU - Larsson, C.
AU - Weber, G.
AU - de Jong, B.
AU - Oosterhuis, J. W.
AU - Molenaar, W. M.
AU - Geurts van Kessel, A.
PY - 1994/3
Y1 - 1994/3
N2 - The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).
AB - The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).
UR - http://www.scopus.com/inward/record.url?scp=0028263579&partnerID=8YFLogxK
U2 - 10.1016/0165-4608(94)90176-7
DO - 10.1016/0165-4608(94)90176-7
M3 - Article
C2 - 8174069
AN - SCOPUS:0028263579
SN - 0165-4608
VL - 73
SP - 11
EP - 16
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -