@article{4fd6d183b12e4c23bf822891bc3dfe87,
title = "Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10",
abstract = "The human excision repair gene ERCC-1 was cloned after DNA mediated gene transfer to the CHO mutant 43-3B, which is sensitive to ultraviolet light and mitomycin-C. We describe the cloning and sequence analysis of the ERCC-1 cDNA and partial characterization of the gene. ERCC-1 has a size of 15 kb and is located on human chromosome 19. The ERCC-1 precursor RNA is subject to alternative splicing of an internal 72 bp coding exon. Only the cDNA of the larger 1.1 kb transcript, encoding a protein of 297 amino acids, was able to confer resistance to ultraviolet light and mitomycin-C on 43-3B cells. Significant amino acid sequence homology was found between the ERCC-1 gene product and the yeast excision repair protein RAD10. The most homologous region displayed structural homology with DNA binding domains of various polypeptides.",
author = "{van Duin}, Marcel and {de Wit}, Jan and Hanny Odijk and Andries Westerveld and Akira Yasui and Koken, {Marcel H.M.} and Hoeijmakers, {Jan H.J.} and Dirk Bootsma",
note = "Funding Information: We are very grateful to Dr. H. Okayama for providing the cDNA library, Drs. L. Prakash and S. Prakash for giving permission to use the RADlO sequence before publication, Dr. A. H. M. Geurts van Kessel for the hybrid panel used for the chromosomal localization, Drs. D. Shaw and T. Hulsebos for the regional chromosomal mapping, P. ten Dijke and R. Kanaar for the excellent help in the experiments, T. Kartasova for contribution in the cDNA sequencing, and Dr. G. C. Grosveld for helpful suggestions. Furthermore, we thank Mrs. R. Boucke for typing the manuscript and Mr. T. van OS for photography. This work was supported by Euratom contract no. BJ6-141-NL and FUNGO, Foundation of Medical Scientific Research in the Netherlands.",
year = "1986",
month = mar,
day = "28",
doi = "10.1016/0092-8674(86)90014-0",
language = "English",
volume = "44",
pages = "913--923",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}