Molecular cloning of a human DNA repair gene

A. Westerveld, J. H.J. Hoeijmakers, M. Van Duin, J. De Wit, H. Odijk, A. Pastink, R. D. Wood, D. Bootsma

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

250 Citaten (Scopus)


Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.

Originele taal-2Engels
Pagina's (van-tot)425-429
Aantal pagina's5
Nummer van het tijdschrift5976
StatusGepubliceerd - 1984
Extern gepubliceerdJa


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