Mutations in potassium channel KCND3 cause spinocerebellar ataxia type 19

Anna Duarri, Justyna Jezierska, Michiel Fokkens, Michel Meijer, Helenius J. Schelhaas, Wilfred F.A. Den Dunnen, Freerk Van Dijk, Corien Verschuuren-Bemelmans, Gerard Hageman, Pieter Van De Vlies, Benno Küsters, Bart P. Van De Warrenburg, Berry Kremer, Cisca Wijmenga, Richard J. Sinke, Morris A. Swertz, Harm H. Kampinga, Erik Boddeke, Dineke S. Verbeek

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

110 Citaten (Scopus)


Objective: To identify the causative gene for the neurodegenerative disorder spinocerebellar ataxia type 19 (SCA19) located on chromosomal region 1p21-q21. Methods: Exome sequencing was used to identify the causal mutation in a large SCA19 family. We then screened 230 ataxia families for mutations located in the same gene (KCND3, also known as Kv4.3) using high-resolution melting. SCA19 brain autopsy material was evaluated, and in vitro experiments using ectopic expression of wild-type and mutant Kv4.3 were used to study protein localization, stability, and channel activity by patch-clamping. Results: We detected a T352P mutation in the third extracellular loop of the voltage-gated potassium channel KCND3 that cosegregated with the disease phenotype in our original family. We identified 2 more novel missense mutations in the channel pore (M373I) and the S6 transmembrane domain (S390N) in 2 other ataxia families. T352P cerebellar autopsy material showed severe Purkinje cell degeneration, with abnormal intracellular accumulation and reduced protein levels of Kv4.3 in their soma. Ectopic expression of all mutant proteins in HeLa cells revealed retention in the endoplasmic reticulum and enhanced protein instability, in contrast to wild-type Kv4.3 that was localized on the plasma membrane. The regulatory β subunit Kv channel interacting protein 2 was able to rescue the membrane localization and the stability of 2 of the 3 mutant Kv4.3 complexes. However, this either did not restore the channel function of the membrane-located mutant Kv4.3 complexes or restored it only partially. Interpretation: KCND3 mutations cause SCA19 by impaired protein maturation and/or reduced channel function.

Originele taal-2Engels
Pagina's (van-tot)870-880
Aantal pagina's11
TijdschriftAnnals of Neurology
Nummer van het tijdschrift6
StatusGepubliceerd - dec. 2012
Extern gepubliceerdJa


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