Myc deletion rescues Apc deficiency in the small intestine

Owen J. Sansom, Valerie S. Meniel, Vanesa Muncan, Toby J. Phesse, Julie A. Wilkins, Karen R. Reed, J. Keith Vass, Dimitris Athineos, Hans Clevers, Alan R. Clarke

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

509 Citaten (Scopus)

Samenvatting

The APC gene encodes the adenomatous polyposis coli tumour suppressor protein, germline mutation of which characterizes familial adenomatous polyposis (FAP), an autosomal intestinal cancer syndrome. Inactivation of APC is also recognized as the key early event in the development of sporadic colorectal cancers, and its loss results in constitutive activity of the β-catenin-Tcf4 transcription complex. The proto-oncogene c-MYC has been identified as a target of the Wnt pathway in colorectal cancer cells in vitro, in normal crypts in vivo and in intestinal epithelial cells acutely transformed on in vivo deletion of the APC gene; however, the significance of this is unclear. Therefore, to elucidate the role Myc has in the intestine after Apc loss, we have simultaneously deleted both Apc and Myc in the adult murine small intestine. Here we show that loss of Myc rescued the phenotypes of perturbed differentiation, migration, proliferation and apoptosis, which occur on deletion of Apc. Remarkably, this rescue occurred in the presence of high levels of nuclear β-catenin. Array analysis revealed that Myc is required for the majority of Wnt target gene activation following Apc loss. These data establish Myc as the critical mediator of the early stages of neoplasia following Apc loss.

Originele taal-2Engels
Pagina's (van-tot)676-679
Aantal pagina's4
TijdschriftNature
Volume446
Nummer van het tijdschrift7136
DOI's
StatusGepubliceerd - 5 apr. 2007
Extern gepubliceerdJa

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