TY - JOUR
T1 - Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells
AU - Giezeman-Smits, Katinka M.
AU - Jonges, Liesbeth E.
AU - Chambers, William H.
AU - Brisette-Storkus, Cynthia S.
AU - Van Vlierberghe, Ronald L.P.
AU - Van Eendenburg, Jaap D.H.
AU - Eggermont, Alexander M.M.
AU - Fleuren, Gert Jan
AU - Kuppen, Peter J.K.
PY - 1998/2
Y1 - 1998/2
N2 - Interleukin-2 (IL-2)-activated natural killer (NK) cells are known to mediate specific functions such as cytolytic activity and tumor infiltration more efficiently than nonactivated NK cells. To investigate whether these characteristics are associated with induction or up-regulation of expression of membrane structures after IL-2 activation, we selected four hybridomas (mAbs ANK44, ANK66, ANK7, and ANK123) derived from mice immunized with rat IL-2-activated NK cells and compared the expression of the epitopes recognized on IL-2-activated NK cells versus unstimulated NK cells. We found that ANK44-expression was induced after activation with IL-2. The antigens recognized by ANK66, ANK7, and ANK123 were expressed selectively on subsets of nonactivated NK cells. After activation with IL-2 the level of expression of these antigens was increased. Moreover, the majority of NK cells then expressed these antigens after IL-2 activation. Biochemical characterization of the membrane structures recognized on IL-2-activated NK cells showed that they have not previously been described. The precise function of these membrane structures is not yet known, however, the selective nature of their expression implies their involvement in the specific functions.
AB - Interleukin-2 (IL-2)-activated natural killer (NK) cells are known to mediate specific functions such as cytolytic activity and tumor infiltration more efficiently than nonactivated NK cells. To investigate whether these characteristics are associated with induction or up-regulation of expression of membrane structures after IL-2 activation, we selected four hybridomas (mAbs ANK44, ANK66, ANK7, and ANK123) derived from mice immunized with rat IL-2-activated NK cells and compared the expression of the epitopes recognized on IL-2-activated NK cells versus unstimulated NK cells. We found that ANK44-expression was induced after activation with IL-2. The antigens recognized by ANK66, ANK7, and ANK123 were expressed selectively on subsets of nonactivated NK cells. After activation with IL-2 the level of expression of these antigens was increased. Moreover, the majority of NK cells then expressed these antigens after IL-2 activation. Biochemical characterization of the membrane structures recognized on IL-2-activated NK cells showed that they have not previously been described. The precise function of these membrane structures is not yet known, however, the selective nature of their expression implies their involvement in the specific functions.
KW - Cytolytic activity
KW - Hybridomas
KW - Tumor infiltration
UR - http://www.scopus.com/inward/record.url?scp=0031907523&partnerID=8YFLogxK
U2 - 10.1002/jlb.63.2.209
DO - 10.1002/jlb.63.2.209
M3 - Article
C2 - 9468279
AN - SCOPUS:0031907523
SN - 0741-5400
VL - 63
SP - 209
EP - 215
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 2
ER -