TY - JOUR
T1 - Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila
AU - Mendjan, Sascha
AU - Taipale, Mikko
AU - Kind, Jop
AU - Holz, Herbert
AU - Gebhardt, Philipp
AU - Schelder, Malgorzata
AU - Vermeulen, Michiel
AU - Buscaino, Alessia
AU - Duncan, Kent
AU - Mueller, Juerg
AU - Wilm, Matthias
AU - Stunnenberg, Henk G.
AU - Saumweber, Harald
AU - Akhtar, Asifa
N1 - Funding Information:
We thank D. Arndt-Jovin, H. Eggert, A. Gortchakov, J. Lucchesi, M. Kuroda, P. Becker, W. Herr, S. Gigliotti, N. Xylourgidis, H. Brock, V. Cordes, B. Papp, and T. Klymenko for providing reagents. We are also grateful to M. Hentze for support and to I. Mattaj, M. Hentze, J. Ellenberg, E. Izaurralde, V. Cordes, D. Zink, A. Ladurner, S. Kass, and members of the lab for critical reading of the manuscript and helpful discussions. K.D. is a recipient of EC Marie Curie Incoming International Fellowship (IIF) under the 6th Framework. J.K. is funded by DFG “SPP1129: Epigenetics.” P.G. is supported by an “E-STAR” fellowship funded by the EC's FP6 Marie Curie Host fellowship for Early Stage Research Training under contract number MEST-CT-2004-504640. This work was supported by the EUFP6 “Epigenome” NoE and SFB “transregio TR5.”
PY - 2006/3/17
Y1 - 2006/3/17
N2 - Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.
AB - Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.
UR - http://www.scopus.com/inward/record.url?scp=33644870525&partnerID=8YFLogxK
U2 - 10.1016/j.molcel.2006.02.007
DO - 10.1016/j.molcel.2006.02.007
M3 - Article
C2 - 16543150
AN - SCOPUS:33644870525
SN - 1097-2765
VL - 21
SP - 811
EP - 823
JO - Molecular Cell
JF - Molecular Cell
IS - 6
ER -