Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J. H. Hooijberg; G. J. Peters; G. J.L. Kaspers; P. R. Wielinga; A. J.P. Veerman; R. Pieters; G. Jansen

doi:10.1081/NCN-200027672

Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J. H. Hooijberg, G. J. Peters, G. J.L. Kaspers, P. R. Wielinga, A. J.P. Veerman, R. Pieters, G. Jansen

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

Originele taal-2	Engels
Pagina's (van-tot)	1451-1454
Aantal pagina's	4
Tijdschrift	Nucleosides, Nucleotides and Nucleic Acids
Volume	23
Nummer van het tijdschrift	8-9
DOI's	https://doi.org/10.1081/NCN-200027672
Status	Gepubliceerd - 2004
Extern gepubliceerd	Ja

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10.1081/NCN-200027672

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title = "Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells",

abstract = "An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.",

keywords = "ABC-transporters, BCRP/ABCG2, Efflux rate, Hoechst 33342, Kol43",

author = "Hooijberg, {J. H.} and Peters, {G. J.} and Kaspers, {G. J.L.} and Wielinga, {P. R.} and Veerman, {A. J.P.} and R. Pieters and G. Jansen",

note = "Funding Information: Supported by the Dutch Cancer Society, grant VU-2000-2237.",

year = "2004",

doi = "10.1081/NCN-200027672",

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volume = "23",

pages = "1451--1454",

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T1 - Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

AU - Hooijberg, J. H.

AU - Peters, G. J.

AU - Kaspers, G. J.L.

AU - Wielinga, P. R.

AU - Veerman, A. J.P.

AU - Pieters, R.

AU - Jansen, G.

N1 - Funding Information: Supported by the Dutch Cancer Society, grant VU-2000-2237.

PY - 2004

Y1 - 2004

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AB - An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

KW - ABC-transporters

KW - BCRP/ABCG2

KW - Efflux rate

KW - Hoechst 33342

KW - Kol43

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AN - SCOPUS:10344222677

SN - 1525-7770

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JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 8-9

ER -

Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

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