TY - JOUR
T1 - Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins
T2 - Targets of Human Amniotic Membrane in Hepatocellular Carcinoma
AU - Mamede, A C
AU - Guerra, S
AU - Laranjo, M
AU - Santos, K
AU - Carvalho, M J
AU - Carvalheiro, T
AU - Moura, P
AU - Paiva, A
AU - Abrantes, A M
AU - Maia, C J
AU - Botelho, M F
N1 - Publisher Copyright:
© 2016, Arányi Lajos Foundation.
PY - 2016/10
Y1 - 2016/10
N2 - The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.
AB - The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.
KW - ATP Binding Cassette Transporter, Subfamily B/metabolism
KW - Amnion/metabolism
KW - Antineoplastic Agents/pharmacology
KW - Apoptosis/drug effects
KW - Carcinoma, Hepatocellular/drug therapy
KW - Cell Cycle/drug effects
KW - Cell Cycle Proteins/metabolism
KW - Cell Line, Tumor
KW - Cyclin-Dependent Kinase Inhibitor p21/metabolism
KW - DNA/metabolism
KW - Hep G2 Cells
KW - Humans
KW - Liver Neoplasms/drug therapy
KW - Membrane Proteins/metabolism
KW - Oxidative Stress/drug effects
KW - Tumor Suppressor Protein p53/metabolism
KW - beta Catenin/metabolism
UR - http://www.scopus.com/inward/record.url?scp=84960423275&partnerID=8YFLogxK
U2 - 10.1007/s12253-016-0053-x
DO - 10.1007/s12253-016-0053-x
M3 - Article
C2 - 26965246
SN - 1219-4956
VL - 22
SP - 689
EP - 697
JO - Pathology oncology research : POR
JF - Pathology oncology research : POR
IS - 4
ER -