Plant homologue of human excision repair gene ERCC1 points to conservation of DNA repair mechanisms

Huiling Xu, Ines Swoboda, Prem L. Bhalla, Anneke M. Sijbers, Chongxin Zhao, Eng Kok Ong, Jan H.J. Hoeijmakers, Mohan B. Singh

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

63 Citaten (Scopus)

Samenvatting

Nucleotide excision repair (NER), a highly versatile DNA repair mechanism, is capable of removing various types of DNA damage including those induced by UV radiation and chemical mutagens. NER has been well characterized in yeast and mammalian systems but its presence in plants has not been reported. Here it is reported that a plant gene isolated from male germline cells of lily (Lilium longiflorum) shows a striking amino acid sequence similarity to the DNA excision repair proteins human ERCC1 and yeast RAD10. Homologous genes are also shown to be present in a number of taxonomically diverse plant genera tested, suggesting that this gene may have a conserved function in plants. The protein encoded by this gene is able to correct significantly the sensitivity to the cross-linking agent mitomycin C in ERCC1-deficient Chinese hamster ovary (CHO) cells. These findings suggest that the NER mechanism is conserved in yeast, animals and higher plants.

Originele taal-2Engels
Pagina's (van-tot)823-829
Aantal pagina's7
TijdschriftPlant Journal
Volume13
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - mrt. 1998
Extern gepubliceerdJa

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